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RAD50 Rabbit pAb

Anti-RAD50 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213727

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213727
Product Name:   Anti-RAD50 Rabbit Polyclonal Antibody
Synonyms:   NBSLD; RAD502; hRad50
UNIPROT ID:   Q92878 (Gene Accession – NP_005723 )
Background:    The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Immunogen:    Synthetic peptide of human RAD50
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using 213727(RAD50 Antibody) at a dilution of 1/40(Nucleus).

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 293T, Hela, K562, NIH/3T3, RAW264.7 cell lysates;
Primary antibody: 213727(RAD50 Antibody) at dilution 1/900;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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