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PAK1 Rabbit pAb

Anti-PAK1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213924

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213924
Product Name:   Anti-PAK1 Rabbit Polyclonal Antibody
Synonyms:   PAKalpha
UNIPROT ID:   Q13153 (Gene Accession – NP_001122092 )
Background:    This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human PAK1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213924(PAK1 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213924(Anti-PAK1 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 213924(Anti-PAK1 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D161067(Anti-PAK1 Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T cells, K562 cells;
Primary antibody: 213924(PAK1 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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