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UPF1 Rabbit pAb

Anti-UPF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S214092

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S214092
Product Name:   Anti-UPF1 Rabbit Polyclonal Antibody
Synonyms:   HUPF1; NORF1; RENT1; smg-2; pNORF1
UNIPROT ID:   Q92900 (Gene Accession – NP_002902 )
Background:    This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human UPF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 214092(UPF1 Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 214092(Anti-UPF1 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 214092(Anti-UPF1 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D161327(Anti-UPF1 Antibody) at dilution 1/35.

Gel: 6%SDS-PAGE, Lysate: 60 μg;
Lane: Raji cells;
Primary antibody: 214092(UPF1 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes
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