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RPF2 Rabbit pAb

Anti-RPF2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215384

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215384
Product Name:   Anti-RPF2 Rabbit Polyclonal Antibody
Synonyms:   BXDC1; bA397G5.4
UNIPROT ID:   Q9H7B2 (Gene Accession – NP_115570 )
Background:    BXDC1 (Brix domain-containing protein 1) is a 306 amino acid protein encoded by the human gene BXDC1. BXDC1 is a nuclear protein that contains one Brix domain. Brix domain containing proteins represent a family of proteins involved in the biogenesis of large ribosomal subunits. The Brix domain is a region with homology to the yeast protein Pitx1 (Ribosome biogenesis protein BRX1). Pitx1 is part of a complex that includes RPF1, RPF2 and SSF1 or SSF2. This complex is required for the biogenesis of the 60S ribosomal subunit.
Immunogen:    Synthetic peptide of human RPF2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 215384(RPF2 Antibody) at a dilution of 1/40(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 215384(Anti-RPF2 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A549, Hela and K562 cell lysates;
Primary antibody: 215384(RPF2 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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