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COX6C Rabbit pAb

Anti-COX6C Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215640

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215640
Product Name:   Anti-COX6C Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P09669 (Gene Accession – NP_004365 )
Background:    Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIc, which has 77% amino acid sequence identity with mouse subunit VIc. This gene is up-regulated in prostate cancer cells. A pseudogene has been found on chromosomes 16p12.
Immunogen:    Synthetic peptide of human COX6C
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 215640(COX6C Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 215640(Anti-COX6C Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 215640(Anti-COX6C Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D163662(Anti-COX6C Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: HEPG2, HUVEC and NIH/3T3 cell, Human heart tissue, Mouse heart tissue, PC-3 cell lysates;
Primary antibody: 215640(COX6C Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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