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AFP Rabbit pAb

Anti-AFP Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216215

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216215
Product Name:   Anti-AFP Rabbit Polyclonal Antibody
Synonyms:   AFPD; FETA; HPAFP
UNIPROT ID:   P02771 (Gene Accession – BC027881 )
Background:    This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Immunogen:    Fusion protein of human AFP
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cardiovascular, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216215(AFP Antibody) at a dilution of 1/60(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216215(Anti-AFP Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 216215(Anti-AFP Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D220001(Anti-AFP Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human liver cancer tissue;
Primary antibody: 216215(AFP Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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