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STAT4 Rabbit pAb

Anti-STAT4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216261

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216261
Product Name:   Anti-STAT4 Rabbit Polyclonal Antibody
Synonyms:   SLEB11
UNIPROT ID:   Q14765 (Gene Accession – BC031212 )
Background:    The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.
Immunogen:    Fusion protein of human STAT4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 216261(STAT4 Antibody) at a dilution of 1/75(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 216261(Anti-STAT4 Antibody) at dilution 1/75.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 216261(Anti-STAT4 Antibody) at a dilution of 1/75.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D220086(Anti-STAT4 Antibody) at dilution 1/75.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: TM4 and 293T cell lysates;
Primary antibody: 216261(STAT4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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