Email: info@neweastbio.com   |  Call: 6109452007

NFKBID Rabbit pAb

Anti-NFKBID Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216288

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216288
Product Name:   Anti-NFKBID Rabbit Polyclonal Antibody
Synonyms:   TA-NFKBH, IkappaBNS
UNIPROT ID:   Q8NI38 (Gene Accession – BC006273 )
Background:    May regulate the expression of IL-2, IL-6, and other cytokines through regulation on NF-kappa-B activity. Functions in the regulation of inflammatory responses. May also regulate TCR-induced negative selection of thymocytes.I?BNS, the nuclear I?B-like protein encoded by Nfkbid, is required for the development of marginal zone and peritoneal B-1 B cells and additionally required for extrafollicular antibody responses to T-independent and -dependent immunogens.I?BNS has been identified as a member of the I?B family of NF-κB inhibitors, which undergoes induction upon TCR signaling. I?BNS mediates both positive and negative gene regulation, depending on individual cell type and/or cytokine.
Immunogen:    Fusion protein of human NFKBID
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216288(NFKBID Antibody) at a dilution of 1/40(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216288(Anti-NFKBID Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 216288(Anti-NFKBID Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D220139(Anti-NFKBID Antibody) at dilution 1/40.

Gel: 10+12%SDS-PAGE, Lysate: 40 μg;
Lane: RAW264.7 cells;
Primary antibody: 216288(NFKBID Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
          Publications