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APIP Rabbit pAb

Anti-APIP Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216290

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216290
Product Name:   Anti-APIP Rabbit Polyclonal Antibody
Synonyms:   APIP2, CGI29, CGI-29, MMRP19, dJ179L10.2
UNIPROT ID:   Q96GX9 (Gene Accession – BC008440 )
Background:    APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury.The protein highly expressed in skeletal muscle and heart and binds to the CARD of Apaf-1 in competition with caspase-9. Exogenous APIP inhibits cytochrome c-induced activation of caspase-3 and caspase-9, and suppresses cell death triggered by mitochondrial apoptotic stimuli through inhibiting the downstream activity of cytochrome c released from mitochondria.
Immunogen:    Fusion protein of human APIP
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 500-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 216290(APIP Antibody) at a dilution of 1/25(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 216290(Anti-APIP Antibody) at dilution 1/25.

Gel: 10%SDS-PAGE, Lysate: 60 μg;
Lane: Mouse heart tissue;
Primary antibody: 216290(APIP Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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