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CAPNS1 Rabbit pAb

Anti-CAPNS1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216382

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216382
Product Name:   Anti-CAPNS1 Rabbit Polyclonal Antibody
Synonyms:   30K, CANP, CDPS, CSS1, CANPS, CAPN4, CALPAIN4
UNIPROT ID:   P04632 (Gene Accession – BC000592 )
Background:    Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. This gene encodes a small subunit common to both calpain I and II and is associated with myotonic dystrophy. Two transcript variants encoding the same protein have been identified for this gene.
Immunogen:    Fusion protein of human CAPNS1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 216382(CAPNS1 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 216382(Anti-CAPNS1 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216382(Anti-CAPNS1 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D220330(Anti-CAPNS1 Antibody) at dilution 1/60.

Gel: 12%SDS-PAGE, Lysate: 60 μg;
Lane 1-2: K562 cells, NIH/3T3 cells;
Primary antibody: 216382(CAPNS1 Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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