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CAPN7 Rabbit pAb

Anti-CAPN7 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216384

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216384
Product Name:   Anti-CAPN7 Rabbit Polyclonal Antibody
Synonyms:   PALBH; CALPAIN7
UNIPROT ID:   Q9Y6W3 (Gene Accession – BC056202 )
Background:    Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The function of the protein encoded by this gene is not known. An orthologue has been found in mouse but it seems to diverge from other family members. The mouse orthologue is thought to be calcium independent with protease activity.
Immunogen:    Fusion protein of human CAPN7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216384(CAPN7 Antibody) at a dilution of 1/20(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216384(Anti-CAPN7 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Human normal liver tissue, mouse liver tissue, mouse intestines tissue, Mouse pancreas tissue, hepg2 cells, HT29 cells, mouse heart tissue;
Primary antibody: 216384(CAPN7 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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