Email: info@neweastbio.com   |  Call: 6109452007

CEACAM8 Rabbit pAb

Anti-CEACAM8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216416

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216416
Product Name:   Anti-CEACAM8 Rabbit Polyclonal Antibody
Synonyms:   CD67; CGM6; CD66b; NCA-95
UNIPROT ID:   P31997 (Gene Accession – BC026263 )
Background:    Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764). By probing white blood cell cDNA libraries with tumor nonspecific crossreacting antigen, Arakawa et al. (1990) isolated a cDNA encoding CEACAM8, which they called NCA-W272. The deduced 349-amino acid protein contains 11 potential N-glycosylation sites and 4 cysteines expected to form 2 intramolecular disulfide linkages. It also has a signal peptide and several domains similar to those of other CEA family proteins, such as CEACAM1 (109770). Immunoblot analysis showed expression of a 70-kD protein that was reduced to a 37-kD major protein and a 34-kD minor protein in the presence of a glycosylation inhibitor.
Immunogen:    Fusion protein of human CEACAM8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216416(CEACAM8 Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216416(Anti-CEACAM8 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 216416(Anti-CEACAM8 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D220405(Anti-CEACAM8 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A431 and HepG2 cell lysates;
Primary antibody: 216416(CEACAM8 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
          Publications