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EMC8 Rabbit pAb

Anti-EMC8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216440

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216440
Product Name:   Anti-EMC8 Rabbit Polyclonal Antibody
Synonyms:   NOC4, COX4NB, C16orf2, C16orf4, FAM158B
UNIPROT ID:   O43402 (Gene Accession – BC001472 )
Background:    The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216440(EMC8 Antibody) at a dilution of 1/30(cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216440(Anti-EMC8 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216440(Anti-EMC8 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D220470(Anti-EMC8 Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, HT-29 cells;
Primary antibody: 216440(EMC8 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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