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RAF1 Rabbit pAb

Anti-RAF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216448

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216448
Product Name:   Anti-RAF1 Rabbit Polyclonal Antibody
Synonyms:   NS5; CRAF; Raf-1; c-Raf; CMD1NN
UNIPROT ID:   P04049 (Gene Accession – BC018119 )
Background:    This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Immunogen:    Fusion protein of human RAF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 216448(RAF1 Antibody) at a dilution of 1/40(Cytoplasm).

Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using 216448(Anti-RAF1 Antibody) at a dilution of 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: HepG2, Mouse fetal muscle tissue, MCF7, NIH/3T3, Ramos, Hela, K562 cell lysates;
Primary antibody: 216448(RAF1 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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