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DKK3 Rabbit pAb

Anti-DKK3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216474

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216474
Product Name:   Anti-DKK3 Rabbit Polyclonal Antibody
Synonyms:   RIG; REIC
UNIPROT ID:   Q9UBP4 (Gene Accession – BC007660 )
Background:    This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Immunogen:    Fusion protein of human DKK3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216474(DKK3 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216474(Anti-DKK3 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216474(Anti-DKK3 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D220554(Anti-DKK3 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal brain tissue;
Primary antibody: 216474(DKK3 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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