Email: info@neweastbio.com   |  Call: 6109452007

DNMT3A Rabbit pAb

Anti-DNMT3A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216476

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216476
Product Name:   Anti-DNMT3A Rabbit Polyclonal Antibody
Synonyms:   TBRS; DNMT3A2; M.HsaIIIA
UNIPROT ID:   Q9Y6K1 (Gene Accession – BC043617 )
Background:    CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Immunogen:    Fusion protein of human DNMT3A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216476(DNMT3A Antibody) at a dilution of 1/80(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216476(Anti-DNMT3A Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 216476(Anti-DNMT3A Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D220559(Anti-DNMT3A Antibody) at dilution 1/80.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and 231 cell lysates;
Primary antibody: 216476(DNMT3A Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
          Publications