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EEF1G Rabbit pAb

Anti-EEF1G Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216487

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216487
Product Name:   Anti-EEF1G Rabbit Polyclonal Antibody
Synonyms:   EF1G, GIG35
UNIPROT ID:   P26641 (Gene Accession – BC013918 )
Background:    This gene encodes a subunit of the elongation factor-1 comple,x which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. Probably plays a role in anchoring the complex to other cellular components.
Immunogen:    Fusion protein of human EEF1G
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216487(EEF1G Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216487(Anti-EEF1G Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human liver cancer tissue;
Primary antibody: 216487(EEF1G Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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