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GRK4 Rabbit pAb

Anti-GRK4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216543

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216543
Product Name:   Anti-GRK4 Rabbit Polyclonal Antibody
Synonyms:   IT11; GPRK4; GRK4a; GPRK2L
UNIPROT ID:   P32298 (Gene Accession – BC117320 )
Background:    This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human GRK4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 216543(GRK4 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 216543(Anti-GRK4 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 216543(Anti-GRK4 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D220722(Anti-GRK4 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T and HUVEC cell lysates;
Primary antibody: 216543(GRK4 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds
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