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HDAC1 Rabbit pAb

Anti-HDAC1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216553

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216553
Product Name:   Anti-HDAC1 Rabbit Polyclonal Antibody
Synonyms:   HD1; RPD3; GON-10; RPD3L1
UNIPROT ID:   Q13547 (Gene Accession – BC000301 )
Background:    Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Immunogen:    Fusion protein of human HDAC1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cardiovascular, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 216553(HDAC1 Antibody) at a dilution of 1/45(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 216553(Anti-HDAC1 Antibody) at dilution 1/45.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T, 231, Jurkat and RAW264.7 cell lysates;
Primary antibody: 216553(HDAC1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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