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MASP1 Rabbit pAb

Anti-MASP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216635

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216635
Product Name:   Anti-MASP1 Rabbit Polyclonal Antibody
Synonyms:   3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
UNIPROT ID:   P48740 (Gene Accession – BC106945 )
Background:    This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human MASP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 216635(MASP1 Antibody) at a dilution of 1/140(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 216635(Anti-MASP1 Antibody) at dilution 1/140.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 216635(Anti-MASP1 Antibody) at a dilution of 1/140.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D220954(Anti-MASP1 Antibody) at dilution 1/140.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human heart tissue and Hela cell lysates;
Primary antibody: 216635(MASP1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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