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MCM5 Rabbit pAb

Anti-MCM5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216638

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216638
Product Name:   Anti-MCM5 Rabbit Polyclonal Antibody
Synonyms:   CDC46; P1-CDC46
UNIPROT ID:   P33992 (Gene Accession – BC003656 )
Background:    The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Immunogen:    Fusion protein of human MCM5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 216638(MCM5 Antibody) at a dilution of 1/40(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 216638(Anti-MCM5 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 216638(Anti-MCM5 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D220960(Anti-MCM5 Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HepG2 cells, K562 cells, Jurkat cells, 231 cells, hela cells;
Primary antibody: 216638(MCM5 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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