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PRCP Rabbit pAb

Anti-PRCP Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216743

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216743
Product Name:   Anti-PRCP Rabbit Polyclonal Antibody
Synonyms:   PCP; HUMPCP
UNIPROT ID:   P42785 (Gene Accession – BC001500 )
Background:    The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen:    Fusion protein of human PRCP
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216743(PRCP Antibody) at a dilution of 1/15(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216743(Anti-PRCP Antibody) at dilution 1/15.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 216743(Anti-PRCP Antibody) at a dilution of 1/15.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D221156(Anti-PRCP Antibody) at dilution 1/15.

Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane 1-3: SKOV3 cells, A549 cells, human liver cancer tissue;
Primary antibody: 216743(PRCP Antibody) at dilution 1/100;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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