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SPDL1 Rabbit pAb

Anti-SPDL1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216804

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216804
Product Name:   Anti-SPDL1 Rabbit Polyclonal Antibody
Synonyms:   CCDC99
UNIPROT ID:   Q96EA4 (Gene Accession – BC012568 )
Background:    This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling.
Immunogen:    Fusion protein of human SPDL1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216804(SPDL1 Antibody) at a dilution of 1/110(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216804(Anti-SPDL1 Antibody) at dilution 1/110.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: K562 cell, Mouse testis tissue, 293T cell lysates;
Primary antibody: 216804(SPDL1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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