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BIRC5 Rabbit pAb

Anti-BIRC5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216815

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216815
Product Name:   Anti-BIRC5 Rabbit Polyclonal Antibody
Synonyms:   API4, EPR-1
UNIPROT ID:   O15392 (Gene Accession – BC008718 )
Background:    This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen:    Fusion protein of human BIRC5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216815(BIRC5 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216815(Anti-BIRC5 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 216815(Anti-BIRC5 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D221289(Anti-BIRC5 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A431 cells, Jurkat cells, hela cells;
Primary antibody: 216815(BIRC5 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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