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TGM3 Rabbit pAb

Anti-TGM3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216834

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216834
Product Name:   Anti-TGM3 Rabbit Polyclonal Antibody
Synonyms:   TGE; UHS2
UNIPROT ID:   Q08188 (Gene Accession – BC109076 )
Background:    Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle.
Immunogen:    Fusion protein of human TGM3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50- 200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216834(TGM3 Antibody) at a dilution of 1/55(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216834(Anti-TGM3 Antibody) at dilution 1/55.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human tongue tissue lysate;
Primary antibody: 216834(TGM3 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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