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ADA Rabbit pAb

Anti-ADA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216933

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216933
Product Name:   Anti-ADA Rabbit Polyclonal Antibody
Synonyms:   ADA1
UNIPROT ID:   P00813 (Gene Accession – BC007678 )
Background:    This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.
Immunogen:    Fusion protein of human ADA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216933(ADA Antibody) at a dilution of 1/60(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216933(Anti-ADA Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 216933(Anti-ADA Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D221495(Anti-ADA Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Raji cell lysate;
Primary antibody: 216933(ADA Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second
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