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AFAP1 Rabbit pAb

Anti-AFAP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216954

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216954
Product Name:   Anti-AFAP1 Rabbit Polyclonal Antibody
Synonyms:   AFAP; AFAP110; AFAP-110
UNIPROT ID:   Q8N556 (Gene Accession – BC032777 )
Background:    The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human AFAP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 216954(AFAP1 Antibody) at a dilution of 1/95(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 216954(Anti-AFAP1 Antibody) at dilution 1/95.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 216954(Anti-AFAP1 Antibody) at a dilution of 1/95.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D221531(Anti-AFAP1 Antibody) at dilution 1/95.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cell lysate;
Primary antibody: 216954(AFAP1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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