Email: info@neweastbio.com   |  Call: 6109452007

ARFGAP1 Rabbit pAb

Anti-ARFGAP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217031

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217031
Product Name:   Anti-ARFGAP1 Rabbit Polyclonal Antibody
Synonyms:   ARF1GAP; HRIHFB2281
UNIPROT ID:   Q8N6T3 (Gene Accession – BC028233 )
Background:    The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human ARFGAP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 217031(ARFGAP1 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 217031(Anti-ARFGAP1 Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T cells, A549 cells, PC3 cells, NIH/3T3 cells;
Primary antibody: 217031(ARFGAP1 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
          Publications