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PGK2 Rabbit pAb

Anti-PGK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217107

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217107
Product Name:   Anti-PGK2 Rabbit Polyclonal Antibody
Synonyms:   PGKB; PGKPS; dJ417L20.2
UNIPROT ID:   P07205 (Gene Accession – BC038843 )
Background:    This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
Immunogen:    Fusion protein of human PGK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gasrtic cancer tissue using 217107(PGK2 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gasrtic cancer tissue is first treated with the fusion protein and then with 217107(Anti-PGK2 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217107(Anti-PGK2 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D221803(Anti-PGK2 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HepG2 cells, human fetal placenta tissue, 293T cells, human testis tissue, NIH/3T3 cells;
Primary antibody: 217107(PGK2 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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