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PIP5K1A Rabbit pAb

Anti-PIP5K1A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217125

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217125
Product Name:   Anti-PIP5K1A Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q99755 (Gene Accession – BC007833 )
Background:    Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha is an enzyme that in humans is encoded by the PIP5K1A gene. Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P.
Immunogen:    Fusion protein of human PIP5K1A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217125(PIP5K1A Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217125(Anti-PIP5K1A Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217125(Anti-PIP5K1A Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D221835(Anti-PIP5K1A Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: PC3 cells, 293T cells;
Primary antibody: 217125(PIP5K1A Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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