Email: info@neweastbio.com   |  Call: 6109452007

HK1/HK2 Rabbit pAb

Anti-HK1/HK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217132

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217132
Product Name:   Anti-HK1/HK2 Rabbit Polyclonal Antibody
Synonyms:   HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase; HKII; HXK2
UNIPROT ID:   P19367/P52789 (Gene Accession – BC021116 )
Background:    Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Immunogen:    Fusion protein of human HK1/HK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217132(HK1/HK2 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217132(Anti-HK1/HK2 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 217132(Anti-HK1/HK2 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D221853(Anti-HK1/HK2 Antibody) at dilution 1/60.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: A431 cells, K562 cells, 231 cells, hela cells, hepG2 cells;
Primary antibody: 217132(HK1/HK2 Antibody) at dilution 1/1200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
          Publications