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ALPP Rabbit pAb

Anti-ALPP Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217184

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217184
Product Name:   Anti-ALPP Rabbit Polyclonal Antibody
Synonyms:   ALP; PALP; PLAP; PLAP-1
UNIPROT ID:   P05187 (Gene Accession – BC009647 )
Background:    There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3′ untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Immunogen:    Fusion protein of human ALPP
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 217184(ALPP Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 217184(Anti-ALPP Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217184(Anti-ALPP Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D221938(Anti-ALPP Antibody) at dilution 1/60.

Gel: 15%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 cells, human placenta tissue;
Primary antibody: 217184(ALPP Antibody) at dilution 1/1550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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