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WNT3A Rabbit pAb

Anti-WNT3A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217286

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217286
Product Name:   Anti-WNT3A Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P56704 (Gene Accession – BC103921 )
Background:    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
Immunogen:    Fusion protein of human WNT3A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience, Signal Transduction, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217286(WNT3A Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217286(Anti-WNT3A Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217286(Anti-WNT3A Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D222111(Anti-WNT3A Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cells;
Primary antibody: 217286(WNT3A Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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