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CYP2E1 Rabbit pAb

Anti-CYP2E1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217320

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217320
Product Name:   Anti-CYP2E1 Rabbit Polyclonal Antibody
Synonyms:   CPE1; CYP2E; P450-J; P450C2E
UNIPROT ID:   P05181 (Gene Accession – BC067435 )
Background:    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
Immunogen:    Fusion protein of human CYP2E1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217320(CYP2E1 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217320(Anti-CYP2E1 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate:40 μg;
Lane 1-3: Mouse fetal liver tissue, Mouse kidney tissue, Mouse liver tissue lysates;
Primary antibody: 217320(CYP2E1 Antibody) at dilution 1/1150;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second
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