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CYP21A2 Rabbit pAb

Anti-CYP21A2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217327

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217327
Product Name:   Anti-CYP21A2 Rabbit Polyclonal Antibody
Synonyms:   CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B
UNIPROT ID:   P08686 (Gene Accession – BC128535 )
Background:    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human CYP21A2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Metabolism, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217327(CYP21A2 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217327(Anti-CYP21A2 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217327(Anti-CYP21A2 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D222185(Anti-CYP21A2 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human breast infiltrative duct tissue;
Primary antibody: 217327(CYP21A2 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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