Email: info@neweastbio.com   |  Call: 6109452007

DCTN1 Rabbit pAb

Anti-DCTN1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217346

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217346
Product Name:   Anti-DCTN1 Rabbit Polyclonal Antibody
Synonyms:   P135; DP-150; DAP-150
UNIPROT ID:   Q14203 (Gene Accession – BC006163 )
Background:    This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Immunogen:    Fusion protein of human DCTN1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 200-1000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217346(DCTN1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217346(Anti-DCTN1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 217346(Anti-DCTN1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D222212(Anti-DCTN1 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 217346(DCTN1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
          Publications