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DCTN2 Rabbit pAb

Anti-DCTN2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217347

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217347
Product Name:   Anti-DCTN2 Rabbit Polyclonal Antibody
Synonyms:   RBP50; DCTN50; HEL-S-77; DYNAMITIN
UNIPROT ID:   Q13561 (Gene Accession – BC000718 )
Background:    This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits.
Immunogen:    Fusion protein of human DCTN2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217347(DCTN2 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217347(Anti-DCTN2 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217347(Anti-DCTN2 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D222213(Anti-DCTN2 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T cells, mouse brain tissue, A375 cells;
Primary antibody: 217347(DCTN2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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