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RNASE3 Rabbit pAb

Anti-RNASE3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217382

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217382
Product Name:   Anti-RNASE3 Rabbit Polyclonal Antibody
Synonyms:   ECP; RNS3
UNIPROT ID:   P12724 (Gene Accession – BC096060 )
Background:    Eosinophil Cationic Protein (ECP) also known as ribonuclease 3 is a basic protein located in the eosinophil primary matrix. In humans, the eosinophil cationic protein is encoded by the RNASE3 gene. ECP is released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body. There are three glycosolated forms of ECP and consequently ECP has a range of molecular weights from 18-22 kDa
Immunogen:    Fusion protein of human RNASE3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 217382(RNASE3 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 217382(Anti-RNASE3 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 217382(Anti-RNASE3 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D222270(Anti-RNASE3 Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: U937 cells;
Primary antibody: 217382(RNASE3 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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