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EEF2 Rabbit pAb

Anti-EEF2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217386

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217386
Product Name:   Anti-EEF2 Rabbit Polyclonal Antibody
Synonyms:   EF2; EF-2; EEF-2; SCA26
UNIPROT ID:   P13639 (Gene Accession – BC126259 )
Background:    This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Immunogen:    Fusion protein of human EEF2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217386(EEF2 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217386(Anti-EEF2 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217386(Anti-EEF2 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D222274(Anti-EEF2 Antibody) at dilution 1/80.

Gel: 6%SDS-PAGE, Lysate:40 μg;
Lane 1-3: Hela, 293T, LOVO cell lyates;
Primary antibody: 217386(EEF2 Antibody) at dilution 1/1350;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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