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FH Rabbit pAb

Anti-FH Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217460

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217460
Product Name:   Anti-FH Rabbit Polyclonal Antibody
Synonyms:   MCL; FMRD; HsFH; LRCC; HLRCC; MCUL1
UNIPROT ID:   P07954 (Gene Accession – BC003108 )
Background:    The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
Immunogen:    Fusion protein of human FH
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217460(FH Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217460(Anti-FH Antibody) at dilution 1/75.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 217460(Anti-FH Antibody) at a dilution of 1/75.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D222390(Anti-FH Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: RAW264.7 cell lysate;
Primary antibody: 217460(FH Antibody) at dilution 1/1150;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds
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