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HSD3B7 Rabbit pAb

Anti-HSD3B7 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217521

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217521
Product Name:   Anti-HSD3B7 Rabbit Polyclonal Antibody
Synonyms:   CBAS1; PFIC4; SDR11E3
UNIPROT ID:   Q9H2F3 (Gene Accession – BC004929 )
Background:    This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human HSD3B7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217521(HSD3B7 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217521(Anti-HSD3B7 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217521(Anti-HSD3B7 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D222512(Anti-HSD3B7 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Skov3 cells;
Primary antibody: 217521(HSD3B7 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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