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IPO4 Rabbit pAb

Anti-IPO4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217542

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217542
Product Name:   Anti-IPO4 Rabbit Polyclonal Antibody
Synonyms:   Imp4
UNIPROT ID:   Q8TEX9 (Gene Accession – BC003690 )
Background:    Importin-4 is a protein that in humans is encoded by the IPO4 gene. Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran.
Immunogen:    Fusion protein of human IPO4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217542(IPO4 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217542(Anti-IPO4 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217542(Anti-IPO4 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222548(Anti-IPO4 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A549 cells, human testis tissue;
Primary antibody: 217542(IPO4 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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