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MECP2 Rabbit pAb

Anti-MECP2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217597

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217597
Product Name:   Anti-MECP2 Rabbit Polyclonal Antibody
Synonyms:   RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
UNIPROT ID:   P51608 (Gene Accession – BC011612 )
Background:    DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development.
Immunogen:    Fusion protein of human MECP2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 217597(MECP2 Antibody) at a dilution of 1/60(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 217597(Anti-MECP2 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217597(Anti-MECP2 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222671(Anti-MECP2 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse lung tissue;
Primary antibody: 217597(MECP2 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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