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MRPL28 Rabbit pAb

Anti-MRPL28 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217612

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217612
Product Name:   Anti-MRPL28 Rabbit Polyclonal Antibody
Synonyms:   p15; MAAT1
UNIPROT ID:   Q13084 (Gene Accession – BC000990 )
Background:    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.
Immunogen:    Fusion protein of human MRPL28
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217612(MRPL28 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217612(Anti-MRPL28 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217612(Anti-MRPL28 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222700(Anti-MRPL28 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T cells, A549 cells, human testis tissue, mouse thymus tissue;
Primary antibody: 217612(MRPL28 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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