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PDHB Rabbit pAb

Anti-PDHB Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217678

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217678
Product Name:   Anti-PDHB Rabbit Polyclonal Antibody
Synonyms:   PDHBD; PHE1B; PDHE1B; PDHE1-B
UNIPROT ID:   P11177 (Gene Accession – BC000439 )
Background:    The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]
Immunogen:    Fusion protein of human PDHB
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 217678(PDHB Antibody) at a dilution of 1/120(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 217678(Anti-PDHB Antibody) at dilution 1/120.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Rat heart tissue, Human heart tissue, HepG2 cell lysates;
Primary antibody: 217678(PDHB Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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