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REC8 Rabbit pAb

Anti-REC8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217748

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217748
Product Name:   Anti-REC8 Rabbit Polyclonal Antibody
Synonyms:   Rec8p; REC8L1; HR21spB
UNIPROT ID:   O95072 (Gene Accession – BC010887 )
Background:    This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion comple,x which regulates sister chromatid cohesion and recombination between homologous chromosomes.
Immunogen:    Fusion protein of human REC8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217748(REC8 Antibody) at a dilution of 1/25(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217748(Anti-REC8 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 217748(REC8 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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