Email: info@neweastbio.com   |  Call: 6109452007

RPA2 Rabbit pAb

Anti-RPA2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217780

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217780
Product Name:   Anti-RPA2 Rabbit Polyclonal Antibody
Synonyms:   REPA2; RPA32; RP-A p32; RP-A p34
UNIPROT ID:   P15927 (Gene Accession – BC001630 )
Background:    Replication protein A 32 kDa subunit is a protein that in humans is encoded by theRPA2 gene. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 217780(RPA2 Antibody) at a dilution of 1/40(Nucleus and Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 217780(Anti-RPA2 Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 231 cells, Jurkat cells, A549 cells, HUVEC cells, Hela cells;
Primary antibody: 217780(RPA2 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
          Publications