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TGM4 Rabbit pAb

Anti-TGM4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217936

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217936
Product Name:   Anti-TGM4 Rabbit Polyclonal Antibody
Synonyms:   TGP; hTGP
UNIPROT ID:   P49221 (Gene Accession – BC007003 )
Background:    Transglutaminase 4 also known as TGM4, TGP or hTGP, is a typical TGase that is specifically expressed in prostate tissue. TGM4 are intercellularly localizing, Ca2+-dependent enzymes, which catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis.
Immunogen:    Fusion protein of human TGM4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217936(TGM4 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217936(Anti-TGM4 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 217936(Anti-TGM4 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D223399(Anti-TGM4 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela, lncap, skov3, 293T and TM4 cell;
Primary antibody: 217936(TGM4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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