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USP5 Rabbit pAb

Anti-USP5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217993

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217993
Product Name:   Anti-USP5 Rabbit Polyclonal Antibody
Synonyms:   ISOT
UNIPROT ID:   P45974 (Gene Accession – BC004889 )
Background:    Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).
Immunogen:    Fusion protein of human USP5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217993(USP5 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217993(Anti-USP5 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217993(Anti-USP5 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D223514(Anti-USP5 Antibody) at dilution 1/80.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: LNCAP cell lysate;
Primary antibody: 217993(USP5 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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