| Cat.#: S218074 |
| Product Name: Anti-S100A16 Rabbit Polyclonal Antibody |
| Synonyms: AAG13; S100F; DT1P1A7 |
| UNIPROT ID: Q96FQ6 (Gene Accession – BC010541 ) |
| Background: The S-100 protein family consists of a group of calcium-binding proteins that are exclusively expressed in vertebrates and exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation and motility of tumor cells. S-100A16 (S100 calcium binding protein A16), also known as AAG13 (aging-associated gene 13 protein), S100F or DT1P1A7, is a 103 amino acid nuclear and cytoplasmic protein that exists as a homodimer that binds one calcium ion per monomer. A member of the EF-hand superfamily, S-100A16 contains two EF-hand domains and is encoded by a gene that maps to human chromosome 1q21.3.The S-100 protein family consists of a group of calcium-binding proteins that are exclusively expressed in vertebrates and exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation and motility of tumor cells. S-100A16 (S100 calcium binding protein A16), also known as AAG13 (aging-associated gene 13 protein), S100F or DT1P1A7, is a 103 amino acid nuclear and cytoplasmic protein that exists as a homodimer that binds one calcium ion per monomer. A member of the EF-hand superfamily, S-100A16 contains two EF-hand domains and is encoded by a gene that maps to human chromosome 1q21.3. |
| Immunogen: Full length fusion protein |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: IHC: 20-100;WB: 500-2000;ELISA: 5000-10000 |
| Host Species: Rabbit |
| Clonality: Rabbit Polyclonal |
| Isotype: Immunogen-specific rabbit IgG |
| Purification: Antigen affinity purification |
| Species Reactivity: Human |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| Research Areas: Signal Transduction |
| Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218074(S100A16 Antibody) at a dilution of 1/20(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218074(Anti-S100A16 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 218074(Anti-S100A16 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D223659(Anti-S100A16 Antibody) at dilution 1/20. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: MCF-7, PC-3 and A431 cell;
Primary antibody: 218074(S100A16 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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